Laboratoire de Bactériologie, Hôpital Central, 29 Avenue du Maréchal de Lattre de Tassigny, 54035 Nancy Cedex, France
Bactericidal synergy was demonstrated against 10 of the 11 isolates tested by time–kill assays. ScienceDirect ® is a registered trademark of Elsevier B.V.© 2017 The Author(s).
Against 16 clinical isolates (two MSSA, seven MSSE, three MRSA and four MRSE) a synergic bacteriostatic effect (FIC index range: 0.2–0.5) between cefepime and vancomycin was observed. Vancomycin used alone was bactericidal for all isolates after 24–48 h of incubation at concentrations of 1–2 × MICs (1–4 mg/L) (Table IBy the chequerboard method, synergy between cefepime and vancomycin was observed for nine (50%) of the 18 methicillin-susceptible staphylococci and for seven (38.8%) of the 18 methicillin-resistant staphylococci.
When methicillin-susceptible isolates were exposed to cefepime alone, a bactericidal effect was noted for all isolates with concentrations of 2 × MIC (1–4 mg/L) at 24 h except for one MSSA isolate (MSSA no. The in vitro activity of cefepime combined with vancomycin was assessed by the chequerboard method against 35 clinical isolates of methicillin-susceptible (MSSA, n = 8) or -resistant (MRSA, n = 10) Staphylococcus aureus and methicillin-susceptible (MSSE, n = 9) or -resistant (MRSE, n = 8) Staphylococcus epidermidis and S. aureus ATCC 25923 (MSSA). In Nye, K. J., Shi, Y. G., Andrews, J. M. & Wise, R. (Oxford University Press is a department of the University of Oxford. Staphylococcus epidermidis (staphylocoque épidermique), Staphylococcus haemolyticus (staphylocoque hémolytique), Staphylococcus saprophyticus (staphylocoque saprophyte).
Among 200 CoNS isolates, Staphylococcus epidermidis was the most prevalent species (87) followed by Staphylococcus haemolyticus (23), ... (Comite´ de L’antibiogramme de la Socie´te´ Franc-aise de Microbiologie Communique´, 1998). Introduction. Although vancomycin is commonly used to treat severe infections caused by methicillin-resistant staphylococci, its use as monotherapy in compromised hosts may be ineffective.
Discrepancies between chequerboard and time–kill methods in the detection of synergy have been reported for several bacterial species including staphylococci.We showed that the combination of cefepime with vancomycin allowed a reduction in the concentrations of vancomycin required to achieve bactericidal activity and induced bactericidal synergy for all methicillin-susceptible and for five of the six methicillin-resistant isolates tested. However, the prolonged and unrestricted use has led to the emergence of mupirocin-resistant (mupR) S. aureus. Synergy was observed more frequently against MSSE (seven of nine isolates) than against MRSE (four of eight isolates).
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Temocillin dosage adjustment in a preterm infant with severe renal disease: a case report
Laboratoire de Bactériologie, Hôpital Central, 29 Avenue du Maréchal de Lattre de Tassigny, 54035 Nancy Cedex, France
© 2001 The British Society for Antimicrobial Chemotherapy
1) for which no bactericidal effect was noted at this concentration. A high rate of ineffective empirical antibiotic therapy for High ceftriaxone minimum inhibitory concentrations were noted for methicillin-susceptible Many UTIs were treated with an empirical antibiotic therapy that was ineffective for We use cookies to help provide and enhance our service and tailor content and ads. However, for four of these strains (two MSSA, one MRSA and one MRSE) the chequerboard method failed to detect synergy.
Staphylococcus saprophyticus is a Gram-positive coccus responsible for uncomplicated lower urinary tract infections (UTIs), mainly in young women (Raz et al., 2005).The resistance of S. saprophyticus to the antibiotics commonly used in the empirical treatment of cystitis, such as cefixime and fosfomycin trometamol, complicates the treatment of patients infected with this … Acute-onset postoperative endophthalmitis occurring 2–7 days after surgery is often caused by Staphylococcus epidermidis, Staphylococcus aureus, and Gram-negative bacilli. In this study, the disks containing 10mg MICs of cefepime ranged from 0.5 to 4 mg/L for MSSE, from 1 to 4 mg/L for MSSA, and from 4 to 128 mg/L for both MRSE and MRSA.